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On the other hand, hyperhomocysteinemia, one of the risk factors of migraine attacks, could be regulated by methyl donors including B6, B9, and B12 (58)

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The samples were submitted to the LC-MS/MS on the same day of Preparation Liquid chromatography (LC) was performed using the following steps: Mobile phase A: 0.2% (v/v) Formic Acid in Water Mobile phase B: 0.2% (v/v) Formic Acid in Acetonitrile Wash solution: Methanol: Acetonitrile: Water= 5:4: 1 (v/v/v) Additional LC parameters are summarized in the following Table: Liquid Chromatography Conditions Employed Data acquisition on the high resolution SCIEX 6600 instrument was performed as follows: • The samples were injected and separated with reversed phase chromatography as described above before been ionized and analyzed with a TripleTOF mass spectrometer